DNA

Part:BBa_K2384001

Designed by: Yi Cai   Group: iGEM17_FAFU-CHINA   (2017-10-16)
Revision as of 09:20, 21 October 2017 by Horace (Talk | contribs)


Lpp-OmpA-MBP(Pb)

Lpp-OmpA-mbp(lead) is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP), which functions as the surface display part to bind lead in the water. The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of OmpA serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None