DNA

Part:BBa_K2384000:Design

Designed by: Yi Cai   Group: iGEM17_FAFU-CHINA   (2017-10-16)
Revision as of 08:58, 21 October 2017 by Horace (Talk | contribs)


T7 RNA polymerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and there is no termination codon with this DNA in pSB1C3 plasmid.


Source

The original sequence of this gene has been learned from the web page of the Nanjing Agricultural University team in 2014 and sequence optimization was carried out through biological company.The optimized sequence is more suitable for the expression in Bacillus megaterium and we add a 6*His tag at the C end to facilitate purification of protein.

References