Part:BBa_K2404016

A TSH antagonist with His-Tags, controlled by the LexA promoter We generated a luciferase construct to test the efficacy of the tobacco leave expression system.

This construct contains the LexA promotor, the luciferase coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A1 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.

A thyroid stimulating hormone antagonist, with His-Tags for purification, under the control of the LexA XVE-inducible promoter native to E. coli . This has the Nopaline synthase gene terminator from Agrobacterium tumefaciens . The construct is contained within the pGB-A2 plasmid. It should be used to produce the TSH antagonist under control of XVE.

Sequence and Features 1-6: BsmBI:
7-348: LexA:
349-2005: LUC+:
2006-2272: NosT:
2282-2287: BsmBI:

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 22
  Illegal PstI site found at 888
  Illegal PstI site found at 930
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 22
  Illegal PstI site found at 888
  Illegal PstI site found at 930
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1143
  Illegal XhoI site found at 955
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 22
  Illegal PstI site found at 888
  Illegal PstI site found at 930
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 22
  Illegal PstI site found at 888
  Illegal PstI site found at 930
  
• 1000
  COMPATIBLE WITH RFC[1000]