Device

Part:BBa_K2246007:Design

Designed by: JUAN CARLOS RUEDA SILVA   Group: iGEM17_TecCEM   (2017-10-12)
Revision as of 14:56, 14 October 2017 by JRUEDA (Talk | contribs) (→‎Design Notes)

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RacI BSLA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 750
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BamHI restriction site as result from clonation into BBa_K2246000. A single mutation is present at at base 645 from the original BBa_K592009 sequence in order to prevent a HinCII cut site.

Source

Synthetic part.

References