Composite

Part:BBa_K2242384

Designed by: YongHao Liang   Group: iGEM17_USTC   (2017-10-12)
Revision as of 11:40, 12 October 2017 by Leung (Talk | contribs)


placI+lacI+pTAC+KmAdh

KMADH is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature. To express this reductase KMADH in Shewallena, we construct a shuttle plasmid. On this plasmid, we use this pTAC to control it’s expression, which is induced by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1353
    Illegal BglII site found at 3620
    Illegal XhoI site found at 3682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None