Part:BBa_K2242419
pTET+ccmA-H
As we have mentioned, it’s the heme attached to the Mtr A and Mtr C that enable them to shuttle electrons. So these two protein belong to a protein family called cytochrome c. For those proteins in this family, they all need to get through a process before they become a mature one, which means they can function as they are meant to, the covalent heme ligation. First, those apo-cytochrome c will be secreted to the periplasm, then a protein complex——ccm A-H will catalyze the process of covalent heme ligation, to attach the heme onto the apo-cytochrom c at the right position, after which our cytochrome c proteins can become mature and function as we expected. In our project, we co-express this ccm A-H protein complex with cytochrome c Mtr CAB to mature Mtr CAB to shuttle electrons from electrode into cytoplasm of our engineered E.coli to increase the synthesis efficiency. We use a constitutively on promoter pTET to control gene ccmA-H’s expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 534
Illegal BamHI site found at 3323
Illegal BamHI site found at 3551
Illegal BamHI site found at 5895 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 188
Illegal AgeI site found at 1015 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 4602
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