Composite

Part:BBa_K2235010:Design

Designed by: Shanlin Tong   Group: iGEM17_Stockholm   (2017-10-11)
Revision as of 12:43, 28 October 2017 by Tongshl (Talk | contribs) (→‎Design Notes)

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Endo beta galactosidase with T7 promoter, RBS and functional unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used to amplify the gblock:

Fwd: CTAGTCTAGAATGTTTGTCTTTATGTTACTATTGCTGCTACCGTTTA

Rev: AAAACTGCAGTTATTCTACAGGAATAAGTTGCCATTGACTTGTCCAATAAGTATTTGGAA

Source

Endo beta galactosidase with T7 promoter, RBS and functional unit

References