Composite
Part:BBa_K2235010
Designed by: Shanlin Tong Group: iGEM17_Stockholm (2017-10-11)
Endo beta galactosidase with T7 promoter, RBS and functional unit
BBa_K2235010 biobrick is a composite of a T7 promoter and RBS followed by endo-β-galactosidase enzyme coding site which is N-terminally attached to a His-tag. This enzyme has been shown to release saccharide chains from glycans expressed in gastric mucus. It performs this action by hydrolysing the bonds next to galactose saccharides in the polysaccharide chains of mucins. The sequence originates from the species Clostridium Perfringens. The basic part BBa_K2235008 consists of the endo beta galactosidase enzyme coding sequence with a His-tag N-terminally attached.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 246
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |