Part:BBa_K2447500:Experience
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In this construct, the photosensitive bacterial protein is constructively produced. In the presence of blue light, EL222 is activated via dimerisation and is able to bind upstream of the pLuxI promoter, thereby allowing the recruitment of RNA polymerase to activate expression of RFP. In the absence of blue light, EL222 does not dimerise and is unable to bind DNA; hence showing minimal RFP expression.
Usage and Characterisation
This part is inserted into pBbE8k backbone and subsequently characterized in E. coli 10Beta and MG 1655. These cells are grown in LB medium (with kanamycin) at 37 degrees to reach OD600 of 0.1 before blue light is administered for 7 hours. For every hour, RFP readings were obtained and cross-compared with the control (no blue light).
Strong RFP expression was shown from both cell types (under blue-light condition) as compared to the control condition where no light was administered. By the 6th hour onwards, RFP productions had tripled for both cell types when compared to the control condition.
In short, this construct could prove useful for any group seeking to elucidate gene expression based on blue light as a physical stimulus.
Applications of BBa_K2447500
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UNIQc48bbd2d3c99da92-partinfo-00000000-QINU UNIQc48bbd2d3c99da92-partinfo-00000001-QINU