Composite
Part:BBa_K2447016
Designed by: Chee Wai Kit (david) Group: iGEM17_NUS_Singapore (2017-10-11)
Phosphate-dependent, temperature-sensitive cascaded system with IM2 expression
This design of this cascade system is similar to our other part BBa_K2447015, with the only exception where the GFP coding sequence is being replaced by E2 immunity protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1026
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2085
Illegal XhoI site found at 1311 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1614
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/biocontainment
//collections/probiotics
//collections/probiotics/biocontainment
None |