Composite

Part:BBa_K2206003:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)
Revision as of 13:31, 8 October 2017 by SLovat (Talk | contribs) (→‎Design Notes)

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Toehold switch for hsa-miR-27b-3p with GFPmut3b


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 758


Design Notes

No terminators are needed as the pSB1C3 plasmid contains terminators.

There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein.

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT

References