Measurement
Part:BBa_K2406053:Experience
Designed by: Jack T. Suitor Group: iGEM17_Edinburgh_UG (2017-09-29)
Revision as of 12:08, 7 October 2017 by JackSuitor (Talk | contribs)
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Applications of BBa_K2406053
This part is used to assay activity of Vika recombinase, whose expression is induced by treating cells with IPTG.
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UNIQe06a8c4c27b2cfb0-partinfo-00000000-QINU UNIQe06a8c4c27b2cfb0-partinfo-00000001-QINU Construct is shown to work through experiment where cells were treated with IPTG and RFP fluorescence was measured. Control cells were not treated with IPTG. Results clearly show RFP is only expressed in presence of IPTG and thus when Vika recombinase is expressed. This confirms function of Vika recombinase, T7-LacO promoter, and Vox target sites.