Coding

Part:BBa_K2485014:Design

Designed by: Michael Herschl   Group: iGEM17_Stanford-Brown   (2017-10-02)
Revision as of 06:34, 27 October 2017 by Mherschl (Talk | contribs) (→‎Design Notes)

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Bio-IcsA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 568
    Illegal AgeI site found at 1045
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2733


Design Notes

A biotinylation site has been included.

Source

This Protein is endogenous to Shigella flexneri.

References