Part:BBa_K2206000

Toehold switch for hsa-miR-15b-5p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region.

This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-15b-5p. In the presence of hsa-mir-15b-5p, translation of the downstream protein coding region occurs.

This part can be used to regulate the expression of any protein in response to the presence of hsa-mir-27b-3p. This part contains a strong RBS.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]