Part:BBa_K2368011

Introduction

Flag+T1R3 overlap

This part is that sweetness receptor T1R3 combines with the FLAG tag.

The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R3 and FLAG tag.

The result of the part is to detect the expression and the location of the T1R3.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]