Part:BBa_K2368015

Introduction

T1R2-GFP

This part is that sweetness receptor T1R2 combines with the GCP tag.

The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R2 and GFP tag.

The result of the part is that the expression of the green fluorescence protein to detect the expression and the location of the T1R2.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]