Composite

Part:BBa_K2379006

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-10-01)
Revision as of 13:24, 1 October 2017 by Ramit (Talk | contribs)


pH sensitive system with RNA pHmeter (wild type)

The RNA pHmeter with natural scar site(BBa_K2379002) is under the regulation of the pLAC promoter(BBa_R0011). Following the RNA pHmeter, the construct contains GFP (BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014).Expression of GFP is regulated by the RNA pHmeter and hence will be produced only at pH 8.4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 173
    Illegal BsaI.rc site found at 906


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Categories
Parameters
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