Composite
Kise Ryota

Part:BBa_K2200006

Designed by: Meredith Eating Xiang   Group: iGEM17_Shenzhen_SFLS   (2017-07-22)
Revision as of 11:27, 28 September 2017 by EatingXiang625 (Talk | contribs)


pU6+sgRNA+pHEf1A+hCas9

This part is consisted of Human U6 promoter, sgRNA that targets the specific BRAF sequence. With its high flexibility of being devised to target different tumor gene sequences, it has a great market upon innovating cancer treatments since it will revolutionize this market by personalizing the remedy.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
    Illegal XhoI site found at 1262
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 997
    Illegal NgoMIV site found at 4214
    Illegal NgoMIV site found at 5123
    Illegal AgeI site found at 375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5242
    Illegal SapI.rc site found at 2633
    Illegal SapI.rc site found at 2875


[edit]
Categories
Parameters
None