Composite

Part:BBa_K2235003:Design

Designed by: Achilleas Fardellas, Hrista Micheva   Group: iGEM17_Stockholm   (2017-09-27)
Revision as of 13:17, 27 September 2017 by Fardellas (Talk | contribs) (→‎Design Notes)


Cumate gene switch expressing BFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 418
    Illegal XhoI site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

There is a multiple cloning site(BamHI, SalI, KpnI, NsiI) between the two expression cassettes. Moreover, XmaI and KasI restriction sites can be found before whereas NdeI and MfeI after the BFP enabling any user to switch to the gene of interest.

Source

Obtained as G-block from IDT and amplified via PCR

References