Composite

Part:BBa_K2217013

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-23)
Revision as of 17:06, 23 October 2017 by MTarek (Talk | contribs)


CAG Promoter

We tried to improve CAG promoter at BBa_K1179069 by making it RFC 10 compatible. CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection
Parameters
function