Measurement

Part:BBa_K2269004

Designed by: José Álvaro Ballesteros González   Group: iGEM17_Valencia_UPV   (2017-09-08)
Revision as of 10:37, 20 September 2017 by Albagoutiel (Talk | contribs)


Register Assembly 1/3 (Luc)

Transcriptional unit composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL). In normal basis, a stuffer fragment will be expressing and only when RDF appears, Ros 1 will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 54
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 54
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None