Tag

Part:BBa_K2333004:Design

Designed by: Xingyu Zheng   Group: iGEM17_William_and_Mary   (2017-06-14)
Revision as of 16:31, 16 June 2017 by CiCi Zheng (Talk | contribs) (→‎Source)


mf-Lon Protein Degradation Tag D (medium)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This specific sequence was created by E. coli codon optimizing the amino acid sequence provided in the supplement by Collins et al, as a DNA sequence was not provided.



Source

This tag was derived using mutagenesis from the endogenous Lon degraded tags from the bacteria Mycoplasma florum by Collins et al. 2014 "Tunable Protein Degradation in Bacteria". This specific sequence was synthesized by IDT.

We use the website www.jcat.de to optimize the codon sequence for E. coli.

References

[1] Collins et al. 2014 "Tunable Protein Degradation in Bacteria" jcat.de was used to codon optimize the sequence for E. coli

[2] Eyal Gur and Robert T. Sauer 2008 "Evolution of the ssrA degradation tag in Mycoplasma: Specificity switch to a different protease"