Tag

Part:BBa_K2333001:Design

Designed by: Ethan M Jones   Group: iGEM17_William_and_Mary   (2017-06-14)
Revision as of 16:19, 16 June 2017 by CiCi Zheng (Talk | contribs) (→‎References)


mf-Lon Protein Degradation Tag A (strong)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This specific sequence was created by E. coli codon optimizing the amino acid sequence provided in the supplement by Collins et al, as a DNA sequence was not provided.


Source

This tag was derived using mutagenesis from the endogenous Lon degraded tags from the bacteria Mycoplasma florum by Collins et al. 2014 "Tunable Protein Degradation in Bacteria". This specific sequence was synthesized by IDT.

References

[1] Cameron DE, Collins JJ. Tunable protein degradation in bacteria. Nature Biotechnology. 2014;32(12):1276–1281. jcat.de *was used to codon optimize the sequence for E. coli