Reporter

Part:BBa_M50057:Design

Designed by: Jack Akerman and Jon Bartlett   Group: Stanford BIOE44 - S11   (2017-04-26)
Revision as of 06:42, 12 June 2017 by Jakerman (Talk | contribs) (→‎References)


Melanin Pigment with 6xHist Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475


Design Notes

The use of Copper and L-tyrosine as precursors for the expression of melanin. We optimized the codons for the E. coli bacteria.


Source

Comes from the Rhizobium etli CFN42 bacteria.

References

http://partsregistry.org/Part:BBa_K274001 "Team:Tokyo Tech/Blackened E. coli." Team:Tokyo Tech/Blackened E. coli. IGEM.org, n.d. Web. 9 May 2017. Lagunas-Muñoz, V.h., et al. 2006. "Optimum Melanin Production Using Recombinant Escherichia Coli." Journal of Applied Microbiology 101(5): 1002-008. Web. “What’s that Stuff?” 1998. Chemical and Engineering News 76(46): 51-56. Web. "Part:BBa_R0010:Experience." Registry of Standard Biological Parts. IGEM, n.d. Web. 9 May 2017. “MOPS EZ Rich Defined Medium.” Teknova: Science Matters. n.d. Web. 10 June 2017. Retrieved from: http://www.teknova.com/EZ-RICH-DEFINED-MEDIUM-KIT-p/m2105.htm “Endy: E. coli Wetern Blot.” 2007. OpenWetWare. Retrieved from: http://www.openwetware.org/wiki/Endy:E._coli_Western_Blot