Signalling

Part:BBa_M50050:Design

Designed by: Anthony Agbay   Group: Stanford BIOE44 - S11   (2016-12-11)
Revision as of 21:15, 11 December 2016 by Aja2488 (Talk | contribs) (→‎Plasmid Map)


IPTG-Inducible LuxS Expression in E. coli for Controlling Growth Rates


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
    Illegal AgeI site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Special Notes

Designing this part required no modifications. During our design process in DNA 2.0, we ran the sequence through codon optimization and tested the restriction sites, but there were no recommendations that required action.


Plasmid Map

BBa M50050 Plasmid Map.png

Source

The source of the LuxS gene came from UniProt. All other parts were provided through DNA 2.0 under the plasmid name PD441-CH.

References