Reporter
GG97

Part:BBa_K2145104:Experience

Designed by: Laura Xinyu Chen, Melody Wu, Katie Maruna   Group: iGEM16_Alverno_CA   (2016-10-14)
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Applications of BBa_K2145104

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Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG97 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

Alverno ca 97.png Alverno ca r 97.png Alverno ca g 97.png

Shown above are OD600 measurements, RFP fluorescence, and GFP fluorescence, respectively, for three clones containing this part in liquid culture. Again, we emphasize that only RFP OR GFP are expressed, but never both. We believe this may be due to supercoiling -- when one gene expresses, it creates supercoils that shut down the other gene.

User Reviews

UNIQ18af0d64852e6784-partinfo-00000000-QINU UNIQ18af0d64852e6784-partinfo-00000001-QINU