Reporter
GG97

Part:BBa_K2145104:Experience

Designed by: Laura Xinyu Chen, Melody Wu, Katie Maruna   Group: iGEM16_Alverno_CA   (2016-10-14)
Revision as of 00:10, 31 October 2016 by Mwu (Talk | contribs)


Applications of BBa_K2145104

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Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG97 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

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User Reviews

UNIQ5ebb7b149089e11f-partinfo-00000000-QINU UNIQ5ebb7b149089e11f-partinfo-00000001-QINU