Reporter
GG97
Part:BBa_K2145104:Experience
Designed by: Laura Xinyu Chen, Melody Wu, Katie Maruna Group: iGEM16_Alverno_CA (2016-10-14)
This experience page is provided so that any user may enter their experience using this part.
Applications of BBa_K2145104
Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG97 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
User Reviews
UNIQ92a6d2863e881550-partinfo-00000000-QINU UNIQ92a6d2863e881550-partinfo-00000001-QINU