Protein_Domain
Part:BBa_K1110003:Experience
Designed by: Lydia Studdard Group: iGEM13_Georgia_State (2013-09-18)
Revision as of 23:19, 29 October 2016 by Cjones84 (Talk | contribs) (→Applications of BBa_K1110003)
Applications of BBa_K1110003
Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. Additionally, further purification of the supernatant was not needed after initial centrifugation. Thus, protein expression of Mambalgin-1 in P. pastoris required a minimal amount of steps.
Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression. Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes.
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