Protein_Domain
Part:BBa_K1952000:Design
Designed by: Alex Dean Group: iGEM16_Kingsborough_NY (2016-10-14)
Revision as of 15:34, 29 October 2016 by Djcamenares (Talk | contribs)
Hydrazine Synthase gamma subunit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 885
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design
This construct contains the coding sequence only, reverse translated from K. stuttgartiensis protein sequence, optimized for expression in E. coli, with subsequent removal of internal restriction sites and repetitive sequences.
Source
K. stuttgartiensis protein sequence; GenBank CAJ73611.1 [1]
The DNA for this part was synthesized by Integrated DNA technologies
References
Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. Nature Vol 479 [doi:10.1038/nature10453]