Coding

Part:BBa_K2066029

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-07)
Revision as of 03:28, 29 October 2016 by Jpmarken (Talk | contribs)


William and Mary iGEM 2016 sequence confirmed this part.

Although we were able to confirm the fluorescence and arabinose inducibility of this construct, we were unable to confirm the repressor functionality in conjunction with BBa_K2066023.

Validation of fluorescence and inducibility: William_and_Mary_29_IC3_Induction.png

pBad - TetR/mCherry Fusion

This is a device which expresses an arabinose-inducible tetR-mCherry fusion protein, in order to simultaneously repress downstream genes while quantifying the level of repressor concentration. The part is on the UNS backbone standard designed by William and Mary iGEM 2016, so the insert is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


[edit]
Categories
Parameters
None