Coding

Part:BBa_K2139002:Experience

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-13)
Revision as of 02:41, 30 October 2016 by Dchristy (Talk | contribs) (→‎Applications of BBa_K2139002)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2139002

T--British_Columbia--gels.png

Figure 1. (top) Western Blot of C. crescentus cellulase expressing strains, ran on SDS-PAGE and blotted with anti-RsaA. Left to right: (1) Thermofisher ladder, (2) Gluc1C cell lysate, (3) Endo5A cell lysate, (4) E1_399 cell lysate, (5) E1_422 cell lysate, (6) negative control ΔGCSS (ΔrsaA) cell lysate, (7) P4A723 (wildtype) cell lysate, (8) Thermofisher ladder, (9) Gluc1C low pH extracted proteins, (10) Endo5A low pH extracted proteins, (11) E1_399 low pH extracted proteins, (12) E1_422 low pH extracted proteins, (13) ΔGCSS (ΔrsaA) low pH extracted proteins, (14) P4A723 (wildtype) low pH extracted proteins. (bottom) SDS-PAGE stained with coomassie brilliant blue, samples loaded in the same order as above for the Western Blot.

British_Columbia_cellulasese_assay.png

Figure 2. Assay for cellulase activity with DNPC substrate.

British_Columbia-Cellulose_growth.png

Figure 3. Cellulase activity and growth assay results for C. crescentus displaying cellulases compared to C. crescentus expressing wildtype RsaA (P4A723).

User Reviews

UNIQ9578b16dff002a0d-partinfo-00000000-QINU UNIQ9578b16dff002a0d-partinfo-00000001-QINU

User Reviews

UNIQ9578b16dff002a0d-partinfo-00000002-QINU UNIQ9578b16dff002a0d-partinfo-00000003-QINU