Generator

Part:BBa_K1915001:Experience

Designed by: Holly Bowman   Group: iGEM16_Georgia_State   (2016-10-05)
Revision as of 01:37, 29 October 2016 by Cjones84 (Talk | contribs) (→‎Applications of BBa_K1915001)


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Applications of BBa_K1915001

  • left|frame|200px|Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction

  • left|frame|Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction.


Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.


Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction.

Western blot of SDS-PAGE with His antibody Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.

Elution 2 exhibited the strongest signal

User Reviews

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