Part:BBa_K2054003:Experience
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Applications of BBa_K2054003
Since the plasmids are made, the next stage we plan to take is to purify the ssDNA out of the cells, using TRIzol® Reagent protocol (Thermo Fisher). With reference to the same article, Elbaz stated that replacing the Zymo-SpinTM IC columns by the Zymo-SpinTM IIC Columns will enhance the binding efficiency.
The following schematic diagram (fig 1a extracted from the article) summarizes the idea of our project idea.
source: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
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