Part:BBa_K2123201

Strong RBS + MerB (Organomercurial Lyase) + Strong RBS + MerA (Mercuric Reductase) + B0015

Overview

This composite part was developed to turn available two mer operon enzymes, related to Hg metabolism: organomercurial lyase (MerB) and mercury reductase (MerA), as you can see below.

<img="https://parts.igem.org/File:UFAM_MERBA_1.png"=>

With this part, you can switch: I) promoter sequences, regulating (or not) by what you want; II) transporters protein, using the preferential one for your chassis; III) and whatever you want related to mercury metabolism! These two enzymes together increase the mercury metabolism spectrum in bacteria, improving bioremediation process. Check how the enzymatic pathway works on “Structure and mechanism” and it’s results in “Usage, Methodology and Experiments”.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 458
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 56
  Illegal NgoMIV site found at 630
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 49