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Part:BBa_K1878005:Experience
Designed by: Margaret Lie Group: iGEM16_Rice (2016-10-14)
Revision as of 07:34, 26 October 2016 by SeraphLuke (Talk | contribs)
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UNIQ30c28e878d7da90d-partinfo-00000000-QINU UNIQ30c28e878d7da90d-partinfo-00000001-QINU
No review score entered. Rice iGEM 2016 |
For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions. |