Composite

Part:BBa_K1893018:Design

Designed by: Henry Lloyd-Laney, Stefan Grossfurthner   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 06:59, 26 October 2016 by HOLL579 (Talk | contribs) (→‎Design Notes)


Arabinose inducible 3-isopropylmalate dehydrogenase (pBAD+leuB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1098


Design Notes

The sequence for leuB was obtained from the NCBI, and no codon optimisation was required, as it is already part of the E. coli genome.

Source

LeuB was amplified from E. coli TURBO cells using PCR. Subsequent rounds of PCR were undertaken to add the B0035 ribosome binding site, and the biobrick prefix and suffix. This block was then ligated into BBa_K1893015

References