Composite

Part:BBa_K2027015:Design

Designed by: Michael Becich   Group: iGEM16_Stanford-Brown   (2016-10-08)
Revision as of 03:28, 26 October 2016 by Mbecich (Talk | contribs) (→‎References)

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Internal Ribosome Entry Site Transcriptional Regulation Mechanism-Theophylline Aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 38
    Illegal BsaI.rc site found at 99
    Illegal BsaI.rc site found at 954


Design Notes

See http://2016.igem.org/Team:Stanford-Brown/SB16_BioSensor_IRES

Source

Ogawa, A. (2011). Rational design of artificial riboswitches based on ligand-dependent modulation of internal ribosome entry in wheat germ extract and their applications as label-free biosensors. RNA, 17(3), 478-488. http://dx.doi.org/10.1261/rna.2433111

References

Sun, H., Zhu, X., Lu, P., Rosato, R., Tan, W., & Zu, Y. (2014). Oligonucleotide Aptamers: New Tools for Targeted Cancer Therapy. Molecular Therapy—Nucleic Acids, 3(8), e182. http://dx.doi.org/10.1038/mtna.2014.32