Coding

Part:BBa_K2127003:Design

Designed by: Nafaa Haddou   Group: iGEM16_IngenuityLab_Canada   (2016-10-14)
Revision as of 00:00, 26 October 2016 by Nhaddou (Talk | contribs) (→‎Source)

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psbT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K2127003 was designed to be synthesized as a gene block and be available for direct use in expression. The part contains a lac promotor as well as a RBS and a double terminator. The coding sequence also contains a double stop codon to ensure that termination of transcription occurs. The sequence is not codon optimized for E.coly bacterial strains but is optimized for cyanobacteria, specifically Synechocystis PCC 6803.

Source

Synechocystis PCC 6803

References