Coding

Part:BBa_K2130005

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-19)
Revision as of 11:11, 24 October 2016 by OOIK0003 (Talk | contribs)


NmCas9

Human codon optimised NmCas9

NmCas9 is originated from Neisseria meningitidis species with a PAM requirement of NGGNGATT that placed at 3' end of spacer sequence. From the results attached, it shows that NmCas9 is generally not performed well for both NHEJ and HDR even with different spacer length that range from 17-23 nucleotides.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 511
    Illegal NgoMIV site found at 3282
    Illegal NgoMIV site found at 3301
    Illegal AgeI site found at 3271
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1373
    Illegal BsaI.rc site found at 2557


[edit]
Categories
Parameters
None