Composite
rAIP

Part:BBa_K2027000

Designed by: Charles Gleason, Michael Becich   Group: iGEM16_Stanford-Brown   (2016-09-07)
Revision as of 10:18, 24 October 2016 by Htale1 (Talk | contribs)


Recombinant Apoptosis-Inducing Protein (L-lysine Alpha Oxidase)

This part is a native Scomber japonicus enzyme codon-optimized for Escherichia coli. The conjugated tag should allow for purification and visualization with anti-FLAG antibodies, visualization with Lumio™ Green, and purification with nickel columns. Tani et al. characterized this enzyme in their quest for synthesis of L-pipecolic acid from racemic lysine after nickel column purification, and we verified the function of our part in a similar way. More information can be found on the experience page for this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1004
    Illegal BglII site found at 1032
    Illegal BamHI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Characterization

[edit]
Categories
Parameters
None