Part:BBa_K2070012:Experience

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[http://2016.igem.org/Team:UT-Tokyo iGEM UT-Tokyo 2016]

Since the Pnrd available in the registry BBa_K847211 is submitted in the RFC[25] standard, the part was redesigned in RFC[10] to deal with the possible disadvantages of RFC[25] standard.

Reconstructed in the RFC[10] format, the Pnrd promoter was ligated in front of part BBa_E0240 and compared to the standard promoter BBa_J23101. The measurement was made approximately 4h after 100 fold dilution in M9 in a 5 min interval. For details of our characterisation, refer to our protocol.

Measurement of nrd Promoter Activity.The fluorescence from the Pnrd promoter is compared with that from the standard promoter BBa_J23101, 3 samples were measured every 9 minutes for each construct. Although there seems to be a slight sudden rise in the promoter activity from the 10-20th minute after the start of measurement, since the change in cell concentration could not be measured simultaneously with fluorescence, it is not certain whether the rise is influenced by the cell cycle/division.

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