Regulatory
pBAD

Part:BBa_K1893015

Designed by: Henry Lloyd-Laney, Stefan Grossfurthner   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 15:03, 23 October 2016 by HOLL579 (Talk | contribs)


Arabinose inducible promoter (pBAD)

A construct for controlling the expression of a gene in front of the pBAD promoter.

Usage and Biology

Without any arabinose in the system, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by RNAP. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of whatever is downstream of the pBAD promoter. We have characterised the activation range of this part by using GFP as a reporter construct. You can find the information here.

It is also possible to switch off the pBAD promoter via catabolite repression once the promoter has been induced by L-arabinose by adding D-glucose to the system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1098


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Categories
Parameters
None