Composite

Part:BBa_K1893010

Designed by: Akash Bhattacharjee, Gear Rotrattanadumrong   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 11:54, 23 October 2016 by AkashB 10 (Talk | contribs)


Constitutive STAR (J23119+STAR)

This is the STAR construct under control of the constitutive promoter j23119. When transcribed, it produces the STAR-antisense RNA (pAD1.A5), which can bind to the sense STAR-target attenuator sequence (pAD1.S5). Thus, only in the presence of STAR-antisense, transcription and expression of coding sequences downstream to the STAR-target is activated.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None