Composite
Part:BBa_K1963007
Designed by: Frank Sargent Group: iGEM16_Dundee_Schools (2016-10-09)
Device for rhamnose-dependent expression of an OsmY.
An assembled device that contains a rhamnose-responsive promoter, a strong RBS and a biobrick encoding the E. coli OsmY protein.
Usage and Biology
Experimental procedures needed.
Figure 1: Whole cell (A) and supernatant blots (B) of prhamnose-OsmY-HA (C) in the presence of increasing concentrations of Rhamnose. Anti-HA immunblot shows whole cell expression and secretion of OsmY-HA in the presence of Rhamnose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/part_collection/2016
None |