Composite

Part:BBa_K1963007

Designed by: Frank Sargent   Group: iGEM16_Dundee_Schools   (2016-10-09)
Revision as of 22:40, 22 October 2016 by Frulhuq (Talk | contribs)


Device for rhamnose-dependent expression of an OsmY.

An assembled device that contains a rhamnose-responsive promoter, a strong RBS and a biobrick encoding the E. coli OsmY protein.

Usage and Biology

Experimental procedures and figure legends needed.


Osmy-hafrulhuq.png

Figure 1: Whole cell (A) and supernatant blots (B) of prhamnose-OsmY-HA (C) in the presence of increasing concentrations of Rhamnose. Anti-HA immunblot shows whole cell expression and secretion of OsmY-HA in the presence of Rhamnose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None