Part:BBa_K1963009
Device for rhamnose-dependent expression of an OsmY fusion to Serratia Hfq.
A rhamnose-regulated device based on existing and new (2016) biobricks. This is designed to induce production of a fusion protein between E. coli OsmY (a secerted protein) and Serratia marcescens Hfq (an RNA binding protein). Co-expression with Serratia sRNA may generate a specific RNA secretion system.
Usage and Biology
Experimental procedures here.
Figure 1. Whole cell blots expressing OsmY-Hfq fusion protein under the control of the rhamnose promoter.
Figure 2. Supernatant blots of OsmY-HA (BBa_K1963007), OsmY-Hfq (E. coli- BBa_K1963008) and OsmY-Hfq (Serratia BBa_K1963009).. Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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