Composite

Part:BBa_K1963009

Designed by: Frank Sargent   Group: iGEM16_Dundee_Schools   (2016-10-09)
Revision as of 22:44, 22 October 2016 by Frulhuq (Talk | contribs)

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Device for rhamnose-dependent expression of an OsmY fusion to Serratia Hfq.

A rhamnose-regulated device based on existing and new (2016) biobricks. This is designed to induce production of a fusion protein between E. coli OsmY (a secerted protein) and Serratia marcescens Hfq (an RNA binding protein). Co-expression with Serratia sRNA may generate a specific RNA secretion system.


Usage and Biology

Experimental procedures here.


Hfq-serratia-frulhuq4.png

Figure 1. Whole cell blots expressing OsmY-Hfq fusion protein under the control of the rhamnose promoter.

Allsupernatant_blots2.png

Figure 2. Supernatant blots of OsmY-HA (BBa_K1963007), OsmY-Hfq (E. coli- BBa_K1963008) and OsmY-Hfq (Serratia BBa_K1963009).. Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None