Composite

Part:BBa_K1962015

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-12)
Revision as of 21:45, 22 October 2016 by Frulhuq (Talk | contribs)


A device for expression of Im-Ia in response to pH

This is a composite part for the production of the Immunity Protein to Colicin Ia in response to environmental pH changes. The composite part consists of a pH sensitive promoter (asr from biobrick BBa_K1231000, an RBS (BBa_B0030) and the Immunity Protein required to neutralise the bacteriocin activity of Colicin Ia (BBa_K1962001).


Usage and Biology

We cloned the Ia-Immunity protein (BBa_K1962001) downstream of a pH sensitive promoter Pasr (BBa_K1231000) and a bile salt sensitive promoter PacrRA (BBa_K1231001) to generate the composite parts (BBa_K1962015) and (BBa_K1962011), respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 215
    Illegal AgeI site found at 422
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None