Coding

Part:BBa_K1962003

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-11)
Revision as of 19:21, 22 October 2016 by Frulhuq (Talk | contribs)


Colicin Ia::Ssp2 Chimera

This part contains an translational fusion protein between the truncated bacteriocin-free version of Colicin Ia (BBa_K1962002) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp2 from Serratia marcescens. Ssp2 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) in Serratia marcescens and it has peptidoglycan endopeptidase activity, degrading peptidoglycan in the periplasm of the target cell where it cleaves between y-D-glutamic acid and L-mesodiaminopimelic acid in peptidoglycan.

Usage and Biology

File:Https://static.igem.org/mediawiki/2016/2/22/T--Dundee--Results23.png

T--Dundee--Results23.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1858
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None