Coding

Part:BBa_K1962007

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-11)
Revision as of 20:13, 22 October 2016 by Frulhuq (Talk | contribs)


Colicin E9::Ssp1 Chimera

This part contains an translational fusion protein between the truncated DNase-domain-lacking version of Colicin E9 (BBa_K1962006) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp1 from Serratia marcescens. Ssp1 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) by Serratia marcescens and it has peptidoglycan endopeptidase activity.


Usage and Biology

Cole9fusionfrulhuq.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1543
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None